NanoElectroPlex™ Technology
Versatile Nanoparticles for Intracellular Delivery of Nucleic Acid
While NanoElectroPlex™ technology is not optimized for in vitro transfection, it is possible to deliver siRNA to cells in culture using a “reverse transfection” protocol in which NanoElectroPlex™ nanoparticles are added to tissue culture wells before cells to be transfected are plated. Using this experimental design we show that NanoElectroPlex™ nanoparticles provide intracellular delivery of an siRNA and specific inhibition of expression of an endogenous gene.
In vitro siRNA inhibition of endogenous gene by NanoElectroPlex™ in tumor cell lines. Tumor cells were reverse-transfected for 24 hr in a 24 well plate with 5 µg/well siRNA formulated in NanoElectroPlex™ and the media replaced every 24 hr. After 72 hr supernatant was removed and assayed for VEGF protein by ELISA. The cells were lysed for total cell protein assay.